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B.Pharm Lab. Instruction Manuals

Pharmacology I

APHE Anatomy, Physiology, and Health Education

Pharmaceutical Analysis

Pharmacy study material

Bones and Skeleton System

Bone disease (Gout) (Rheumatoid arthritis) (Osteoarthritis) (Osteoporosis)

Cancer and music therapy

Memory of water

3.5.3 Pharmacology I

swiss albino rat

Lab 12 Collection of blood from orbital sinus of rats and separation of RBC

Theory

Blood is the body tissue which can be easily taken out from the body. That is the reason blood profiles are taken into account to diagnose a diseases, progression of the disease condition or several bioassays testing (like preliminary screening, dose fixing, toxicity study, comparative efficacy study, impact of drug on blood cells count, hormone concentrations, immunity etc.).

For the purpose of bioassay and drug screening, the in vivo experiments are done, commonly using blood of rats, mice, rabbits etc. We can take out only 1% of body weight in every two weeks. If less than the maximum amount of blood is withdrawn, the body replaces blood constituents at the rate of 1 ml/kg/day. The given table represents the amount of blood could be removed in one week from different animals:

Table 1 Representation of blood collection volume of common laboratory animals

Animals Body weight(gm) Blood vol/2 weeks(ml)
Mice 20 0.2
Rats 100 1.0
Rabbits 3500 3.5

We can collect the blood from different site of an animal which are mentioned in the given table:

Table 2: Guidelines for Blood Collection sites

Animals Blood collection sites
Mice Lateral tail vein, Orbital Sinus or Plexus, Lateral Saphenous Vein, Submandibular or Facial Vein, Tail Snip Technique, Cardiac Puncture
Rats Lateral tail vein, Orbital Sinus or Plexus, Lateral Saphenous Vein, Jugular Vein, Anterior Vena Cava, Cardiac Puncture
Rabbits Marginal/ Central Ear Vein, Lateral Saphenous Vein, Cephalic Vein, Jugular Vein, Anterior Vena Cava, Cardiac Puncture

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